WebJun 1, 2009 · Transfection of MC3T3-E1 cell line was carried out using Targefect-2 and Virofect enhancer (Targeting Systems, Santee, CA) when confluency was between 70% and 90%. Human embryonic kidney (HEK)-293 cells (American Type Tissue Collection, passages 2 – 10 ) were grown in DMEM media (Invitrogen) containing 4.5 g/l d -glucose and were … WebTargefect-HUVEC (Targefect F-2 & virofect, peptide enhancer) For transfection of Human, bovine, porcine endothelial cells, microvascular endothelial cells, Human aortic …
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WebJan 18, 2011 · To investigate the influence of medium and transfection time, primary hepatocytes were transfected with Metafecte Pro, Lipofectamine 2000 or Targefect in the presence of DMEM (Figure 2A) or Opti-MEM, with/without Virofect enhancer, for 3 hours or 16 hours (Figure 2A, 2B, 2C).The presence of Opti-MEM resulted in significantly higher … WebThe Targefect-BAC reagent works by itself to transfec BACs into HEK-293 cells but in many other cell types the use of an enhancer (Peptide Enahncer or Virofect ) is required for BAC … WebNational Center for Biotechnology Information bleacher report watch live