WebThe mini binary vector has been demonstrated to successfully transform Arabidopsis plants. Based on this mini binary vector, a series of binary vectors were constructed for plant transformation. Download to read the full article text References An, G., Watson, B., Stachel, S., Gordon, M.P. and Nester, E.W. 1985. WebThe pCambia1301 vector offers: High copy number in E.coli for high DNA yields. pVS1 replicon for high stability in Agrobacterium. Small size. Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest. Bacterial selection with kanamycin. Plant selection with hygromycin B.
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WebDNA that is inserted between the T-DNA borders will be efficiently transferred to and stably maintained within the plant genome. Keywords. Binary Vector; Fall Leaf Lake; Callus … WebpCAMBIA1302. Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GFP genes. Sequence Author: Cambia. Open in SnapGene. Try … scribing worktops
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WebThe T-DNA portion of the binary vector is flanked by left and right border sequences and may include a transgene as well as a plant selectable marker. Outside of the T-DNA, the … WebAgrobacterium tumefaciens and related Agrobacterium species are well-known plant pathogens that have been engineered to efficiently transfer specific pieces of DNA (called transfer DNA, or T-DNA) into the plant nucleus using binary vector systems, which consists of a T-DNA binary vector and a vir helper plasmid. WebApr 29, 2024 · The gene coding for the putative 54-kDa replicase gene of CFMMV was cloned into an Agrobacterium tumefaciens binary vector, and transformation was performed on cotyledon explants of a parthenocarpic cucumber cultivar. ... The suitability of AG as an expression vector in plants was tested by analysis of two infectious viral constructs, each ... scribing wood trim