Phospholipidosis assay

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August undefined, 2024

WebinstaCELL® Phospholipidosis Assay Kits. Drug induced accumulation of phospholipids (phospholipidosis) is associated with clinical toxicities. Cell‐based assays can help to … Webcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a

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WebTo identify compounds that induce intracellular phospholipidosis, we used a high-content imaging method to optimize and validate the cell-based PLD assay. The assay was … WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. … how much should college students study

High content screening analysis of phospholipidosis

WebSuch assays rely heavily on the development prospective assays aim to predict toxicities that could lead to the ter- of human physiologically relevant model systems in combination with mination of a drug candidate or an entire development programme, appropriate toxicity end points. and bespoke retrospective assays are used for issue resolution ... WebNational Center for Biotechnology Information WebFeb 1, 2007 · Phospholipidosis was determined by algorithms that evaluated the granular texture of the perinuclear area as an indicator of the amount of phospholipidosis vacuoles. ... High-content screening... how do taoists find inner peace

Validation of an in vitro screen for phospholipidosis using a high ...

In Vitro Detection of Drug-Induced Phospholipidosis Using Gene ...

WebMembrane permeability assays demonstrate that the alkaloids are biologically available, although fangchinoline showed lower permeability than tetrandrine. At high concentrations, the extract, alkaloid fractions, and pure alkaloids induced phospholipidosis in 293TAT cells and less so in VeroAT cells. Gene expression profiling during virus ... WebJul 7, 2004 · Required concentrations for increased Nile Red binding were generally three-fold or more lower than the cytotoxic concentration determined by a resazurin reduction assay in the same cells. Qualitatively similar Nile Red binding results were obtained when primary canine or rat hepatocytes were used. how do tankless water heaters workWebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high … how much should chimney cleaning cost

"WebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … " - Phospholipidosis assay

Phospholipidosis assay

HCS and HCA Sample Data Thermo Fisher Scientific - US

WebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a leading cause of attrition during drug development.

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WebAug 2010 - Oct 20144 years 3 months. Jalandhar,Punjab,India. The active area of research at University was Metabolic disorder (Diabetes).I was involved in in-vitro selection & characterization, target validation for metabolic targets, assay development and condition optimization, its high throughput screening, selection of hits among phyto ... WebDec 1, 2011 · Phospholipidosis is a lipid storage disorder which is recognized by excessive accumulation of phospholipids in the lysosomes within cells. Phospholipidosis can result from hereditary predisposition, which is for example the case in Niemann-Pick disease, but may also be the result of drug exposure.

WebPhospholipidosis (PLD) is a lysosomal storage disorder induced by compounds, notably cationic amphiphilic drugs, which although reversible interferes with cellular … WebDec 1, 2011 · In summary, we demonstrated an in vitro phospholipidosis screening assay in CHO-K1 and HepG2 cells based on a high content screening analysis method that can …

WebMar 5, 2015 · Synthesis and phospholipidosis effect of a series of cationic amphiphilic compounds: a case study to evaluate in silico and in vitro assays Article Full-text available WebWhen studying phospholipidosis/steatosis in vitro, IONTOX uses HepG2 cells in combination with LipidTox Red, as our primary model to discriminate between phospholipidosis …

WebeCiphr ® Cardio — iPS cell-derived cardiomyocytes and MEA. Structural cardiotoxicity assay — using 3D spontaneously beating microtissues. Combined hypertrophy and cardiotoxicity assay — using 3D spontaneously beating microtissues. Cardiotox screen — …

Web+ selectively discriminates phospholipidosis from steatosis assay ready HepG2 cells assay ready HepG2 cells have an instantly high viability, low debris and attach within 24 hours. drug induced phospholipidosis assay ready HepG2 were treated for 48 hours with Sertraline, a potent inductor of phospholi-pidosis, and Cyclosporine A for negative ... how much should closing costs be on refinanceWebIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H 2 … how much should corrugated sheets overlapWebLC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without preincubation with a mixture of … how much should closing costs be for mortgageWebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high ... how much should contributions to 401k beWebMar 24, 2024 · Drug-induced phospholipidosis: Human: ASCs: Matrigel: Expression of CYP450 enzyme (CYP3A4); ... (LoT) assay to analyze the hepatoxicity of 238 marketed drugs (32 negative controls and 206 reported DILI agents) and showed high predictive values of the LoT system with 88.7% sensitivity and 88.9% specificity. how much should concrete work costWebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. how much should curtain pole overlap windowWebJul 2, 2008 · In vitro screening assays were developed in HepG2 cells, rat primary hepatocytes, and rhesus monkey hepatocytes using the fluorescent-labeled phospholipid probe N - (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (NBD-PE) or Nile Red lipid stain. how much should closing costs be on a house