Ctcf shrna

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August undefined, 2024

WebGenetic locus: CTCF (human) mapping to 16q22.1. PRODUCT CTCF siRNA (h) is a pool of 3 target-specific 19-25 nt siRNAs designed to knock down gene expression. Each vial contains 3.3 nmol of lyophilized siRNA, sufficient for a 10 µM solution once resuspended using protocol below. Suitable for 50-100 transfections. Also see CTCF shRNA Plasmid …

SANTA CRUZ BIOTECHNOLOGY, INC. CTCF siRNA (h): sc-35124

WebSep 1, 2016 · In CTCF-shRNA cells, CTCF expression was further diminished and PAX6, ADAM17 and RCN1 protein expressions were enhanced following the time course. Effects of altered CTCF activity on PAX6 and PAX6-associated ADAM17 and RCN1 expression were further measured by RT-PCR in both Lv-control and CTCF-shRNA HTCE cells. WebMar 8, 2024 · Depletion of endogenous CTCF in the BMDCs was confirmed at the mRNA and protein levels (Fig. 1b, c).Genome-wide CTCF binding patterns, measured by ChIP … greenest energy company uk

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WebNov 5, 2012 · Mutation of CTCF-binding sites in plasmid-borne promoters reduced transcriptional activity in an orientation-dependent manner. Depletion of CTCF by … WebApr 10, 2024 · In parallel, an unbiased in vivo shRNA screen to identify regulatory transcription factors of memory CD8 + T cell differentiation further confirmed the role of … WebJun 1, 2015 · CTCF is a key regulator of nuclear chromatin structure, chromatin organization and gene regulation. The impact of CTCF on transcriptional output is quite varied, ranging from repression, to transcriptional pausing and transactivation. ... The mouse B lymphocyte cell lines Wehi-231 expressing shRNA construct Control (Wehi-CT) or a shRNA ... fluid drams to oz

DNA architectural protein CTCF facilitates subset-specific …

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WebDec 26, 2024 · Efficient CTCF knockdown and single cell RNA-seq. We knocked down CTCF in EL4 cells by short hairpin RNA (shRNA). Western blotting showed a dramatic … WebNSD2 Low cell lines were generated in these three B-ALL cell lines because they naturally harbor a heterozygous NSD2 EK mutation. More specifically, a short-hairpin RNA (shRNA: GGAAACTACTCTCGATTTATG) was used to knockdown NSD2 targeting the NSD2 Type II and NSD2 RE-IIBP isoforms both of which contain the SET domain. fluid drainage from heartWebMay 5, 2024 · Deletion of CTCF attenuated adipogenesis while overexpression enhanced adipocyte differentiation, indicating CTCF is indispensable for adipogenesis. ... Sequences of shRNA oligos can be found in Supplemental Table 1. Lentiviral vectors carrying ATF4 or CTCF cDNAs were purchased from Addgene (USA) or GeneCopoeia (China). PPARγ … greenest european country

"WebCTCF (the CCCTC-binding factor) is a sequence-specific DNA binding protein that recognizes extended DNA motifs with its 11 zinc fingers and acts as a critical regulator of interphase genome folding (Splinter et al ... (NT) shRNA. (G) Effects of 2 h of LPS stimulation on the 7543 H3K9me3 blocks methylated by SETDB1. (H) ... " - Ctcf shrna

Ctcf shrna

CTCF Expression is Essential for Somatic Cell Viability and …

WebMar 29, 2012 · This shRNA knockdown was confirmed by qPCR with primers specific for CTCF (Fig. 6B). Expression of CTCF shRNA resulted in a significant decrease in … WebNov 30, 2024 · Inhibition of cell proliferation and clonogenicity following CTCF shRNA knockdown in K562 cells. (A) Immunoblot of CTCF shRNA knockdown in the presence and absence of doxycycline (dox) compared to ...

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WebFeb 20, 2024 · First, we performed RNA-seq analysis to compare the expression profiles of CTCF shRNA and control shRNA transfectants (raw data accessible via GSE169451) (Figure S6A–E) and identified 2110 upregulated genes and 1908 downregulated genes ( Fold Change > 1.5, q < .05) after knockdown of CTCF in TAMs (Figure 3B). WebSep 1, 2016 · Furthermore, CTCF activities were altered by CTCF-shRNA to study the effect of CTCF on mediating interaction of Pax6 and differentiation-associated genes in corneal epithelial cell fate.

WebNational Center for Biotechnology Information WebJul 20, 2024 · Using shRNA targeting CTCF to recapitulate CTCF haploinsufficiency, we have identified a novel role for CTCF in the regulation of cellular polarity of endometrial glandular epithelium. Overall, we have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial ...

WebApr 13, 2015 · B). Knockdown of CTCF with shRNA blocks viral genome amplification. CIN612 were infected with lentiviruses encoding shRNA to CTCF and after 48 hours total cell extracts were isolated and analyzed by Western blot for levels of CTCF. shRNA knockdown of CTCF blocks HPV-31 differentiation-dependent viral amplification as … WebMay 21, 2024 · As shown in Figs 5C and S11, CTCF and control shRNA expressing cells showed very similar profiles of tax mRNA and RFP production. Taken together, these results suggest that loss of CTCF binding at the vCTCF-BS leads to increased viral gene expression, and that this effect is not due to loss of CTCF binding elsewhere in the host …

WebUsing 3C-based experiments in B cells where CTCF protein levels were downregulated by shRNA-mediated CTCF depletion or Mb1-Cre-mediated conditional deletion of the Ctcf gene in vivo, we and others have shown that in the absence of CTCF IgH locus contraction is only modestly affected (Degner et al., 2009) and Igκ long-range chromatin ...

WebCTCF is a zinc modifying complexes, such as p300, HDACs, PRMT1 and finger protein implicated in insulator function in vertebrate polycomb complexes (15,19). YY1 is evolutionarily well con- species, and the CTCF binding sites located within H19-ICR served throughout the vertebrate and invertebrate lineages have been shown to function as an ... fluid distribution and gas adsorptionWebMay 23, 2011 · At 3 d after knockdown, the percentage of viable GFP + cells was the same in the cells transduced with either control or CTCF shRNA containing retroviruses. As the cells progress through days 4 and 5, the percentage of GFP + cells drops slightly in cells in which CTCF has been knocked down. Thus, it seems that as the cells completely lose … fluid distribution systemWebDec 26, 2024 · Results: We knocked down CTCF in EL4 cells using shRNA, and conducted single cell RNA-seq on both wild type (WT) cells and CTCF-Knockdown (CTCF-KD) cells using Fluidigm C1 system. Principal component analysis of single cell RNA-seq data showed that WT and CTCF-KD cells concentrated in two different clusters on PC1, … greenest family suvWeb网狐最新6603源码.rar. 最新网狐最新6603源码开源代码精品学习vc不可错过的好代码网狐代码是每个学习vc并且迅速成为vc开发高手的不可多得的绝对精品,不说了,对于学习者,得网狐代码你就幸运,舍网狐代码你就再浪费声明二三年 greenest family of the yearWebFeb 22, 2024 · transfected with short hairpin RNA (shRNA)-CTCF, pLenti-CMV-CTCF, shRNA-POLD1, and pLenti-CMV-POLD1, and the results showed that the CTCF may contribute to the altered expression of POLD1 in aging. fluid drainage from thoracentesis normalWebAn unenriched total input fraction was processed for comparison. bShort hairpin mediated CTCF knockdown in two separate shRNA targeting CTCF ve rified by western blotting after 3 and 5days of shR NA induction including shRNA non-silencing contr ol (shNSC). Data shown are one representative of 3 independent experiments using immortalized HPECs. greenest home printer most sustainableWebApr 10, 2024 · In parallel, an unbiased in vivo shRNA screen to identify regulatory transcription factors of memory CD8 + T cell differentiation further confirmed the role of CTCF in CD8 + T cell differentiation (Figures S3B and S3C; Tables S4 and S5). shRNA constructs targeting CTCF were enriched in the T EM and T CM populations … fluid dram to oz