Cs2-cmv-mcs-ires2-bsd
WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebJan 21, 2024 · Human TMPRSS2 and ACE2 genes were cloned into the self-inactivating lentiviral vector plasmids, CSII-CMV-MCS-IRES2-Bsd (RIKEN BRC) and pLVSIN-CMV Pur (Takara Bio), respectively. The resulting constructs were named CSII-CMV-TMPRSS2-IRES2-Bsd and pLVSIN-CMV-ACE2-Pur. For lentiviral vector preparation, 293T cells …
Cs2-cmv-mcs-ires2-bsd
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WebOct 20, 2024 · Scan and upload a completed, printed and signed MCS-150 to our web form (this is the best option and will provide you with a tracking number for your submission) … WebJan 20, 2024 · To establish VMO1-overexpressing HT1080 cell line, the CSII-CMV-MCS-IRES2-Bsd-VMO1 or CSII-CMVMCS-IRES2-Bsd-luciferase plasmid was transfected into HEK293T cells using the Lentivirus High Titer Packaging Mix (Takara Bio Inc., Shiga, Japan) for lentivirus production. After 6 h of transfection, the cells were washed with PBS and …
WebThis work was supported by a grant from the Japan Agency for Medical Research and Development (AMED) under Grant Number JP17gm5810010 to S.F. and by Core Research for Evolutional Science and Technology (CREST) from Japan Science and Technology (JST) under Grant Number JPMJCR14W4 to K.N., R.Y., and T.M.; by Grants-in-Aid for …
WebMay 18, 2024 · RDB04383. Resource name. CSII-CMV-MCS-IRES2-Venus. Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker. Comment. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is … WebOct 11, 2024 · (d) Approximately 1.5 × 10⁶ HEK293T cells were co-transfected with 0.4 μg of pHCMV-VSV-G, 0.9 μg of pCMV deltaR8.2 and 1.4 μg of pCSII-CMV-luc-IRES2-Bsd together with either EV or increasing ...
Webcloned into the CSII-CMV-MCS-IRES2-Bsd vector (RIKEN BioResource Research Center) between the NhelI and EcoRI restriction enzyme . 202 AND ET A L. sites with FLAG tag. The blank vector or CSII-CMV-GFP-IRES2-Bsd vector was used as control for lentiviral infection. These plasmids were transfected into H23 and H441 cell lines using HilyMax ...
WebThird-generation lentiviral PrecisionShuttle™ vector with a blasticidin resistance marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags. shannon mcdonald attorneyWebCs2 cmv mcs ires2 bsd. Cs2 cmp upgrade 2024. Cs2 cmv rfa ires venus. Cs2 cmp upgrade. Checkout Keyword Suggestion with other keyword: Show result. Domain Informations. Whois Informations; Top 10 Domain Provider; Administrator. name : Le Anh Tuan; handle : LAT4-AP; email : [email protected] shannon mcdonald artistWebSep 20, 2024 · With its latest release of SpecLink Cloud, RIB introduces new formatting and export options to support owners, architects, engineers, and specifiers who need a … shannon mcdonald komo weatherWebCMV R U5 5' SD RRE 3' SA cPPT+CTS CMV MCS IRES2 Bsd Rdel U3 PRE U5 BGH pA f1 ori SV40 pro & ori Zeo SV40 pA ColE1 ori Amp 1. Plasmid name: CSII-CMV-MCS-IRES2 … shannon mcdonald obituaryWebFeb 3, 2024 · The CSII-CMV-MCS-IRES2-Bsd-GFP plasmid was used as a control (GFP). (B) Cells were stained for F-actin with rhodamine phalloidin. Bar, 10 μm. (C, D) Cells were seeded on Matrigel-coated wells (1.8 × 10 4 cells per well). Images were captured at the indicated times after seeding (C), and tube numbers were counted in four random fields (D). shannon mcdonald fnhaWebCsii Ef Mcs Ires2 Venus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... pCDNA3 WT B-Myb Johnson et al., 1999 Addgene Plasmid #25965 Plasmid: pRL-CMV Promega Cat#E2261 Software and Algorithms GraphPad Prism (version 7) GraphPad RRID: SCR_002798 Microsoft Excel … shannon mcdowell law officeWebThe .gov means it’s official. Local, state, and federal government websites often end in .gov. State of Georgia government websites and email systems use “georgia.gov” or “ga.gov” … shannon mcdonald wilton ct